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Odyssey Imaging and EMSA


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#1 lizbit

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Posted 22 July 2010 - 09:50 AM

Hello all,

I am running an EMSA and had it recommended to me to use an Odyssey Imaging device that is available for my use on campus.

My problem is that the area I am targeting is not one of the pre-made oligos available through LI-COR. Does anyone know if I can order primers through invitrogen (or any other company, I'm not terribly picky!) and label it that way? Can I order it directly from invitrogen with a label? I've looked all over the LI-COR site and can't find any hint of a labelling kit for oligos.

Thanks in advance!
Liz

#2 Mortis

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Posted 22 July 2010 - 07:14 PM

Im not sure about Invitrogen, but ive ordered biotin and dig labeled primers for EMSA from Sigma before. Theyre fairly expensive though.

Hope that helps

Hello all,

I am running an EMSA and had it recommended to me to use an Odyssey Imaging device that is available for my use on campus.

My problem is that the area I am targeting is not one of the pre-made oligos available through LI-COR. Does anyone know if I can order primers through invitrogen (or any other company, I'm not terribly picky!) and label it that way? Can I order it directly from invitrogen with a label? I've looked all over the LI-COR site and can't find any hint of a labelling kit for oligos.

Thanks in advance!
Liz



#3 bob1

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Posted 25 July 2010 - 03:07 PM

For the Odessey you need their particular moelcules that are detectable by IR lasers, I don't think Invitrogen or Sigma produce the molecules (they will be proprietary to LI-COR), so you won't have much luck there. I can't remember if you can use the camera on the Odessey system for ordinary wavelenght detection or not, it would pay to check the manual or contact the LI-COR help desk to ask them about it.




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