2 replies to this topic

[lizbit]

Hello all,

I am running an EMSA and had it recommended to me to use an Odyssey Imaging device that is available for my use on campus.  

My problem is that the area I am targeting is not one of the pre-made oligos available through LI-COR. Does anyone know if I can order primers through invitrogen (or any other company, I'm not terribly picky!) and label it that way? Can I order it directly from invitrogen with a label? I've looked all over the LI-COR site and can't find any hint of a labelling kit for oligos.

Thanks in advance!
Liz

[Mortis]

Im not sure about Invitrogen, but ive ordered biotin and dig labeled primers for EMSA from Sigma before. Theyre fairly expensive though.

Hope that helps

lizbit, on Jul 23 2010, 03:50 AM, said:

Hello all,

I am running an EMSA and had it recommended to me to use an Odyssey Imaging device that is available for my use on campus.  

My problem is that the area I am targeting is not one of the pre-made oligos available through LI-COR. Does anyone know if I can order primers through invitrogen (or any other company, I'm not terribly picky!) and label it that way? Can I order it directly from invitrogen with a label? I've looked all over the LI-COR site and can't find any hint of a labelling kit for oligos.

Thanks in advance!
Liz

[bob1]

For the Odessey you need their particular moelcules that are detectable by IR lasers, I don't think Invitrogen or Sigma produce the molecules (they will be proprietary to LI-COR), so you won't have much luck there.  I can't remember if you can use the camera on the Odessey system for ordinary wavelenght detection or not, it would pay to check the manual or contact the LI-COR help desk to ask them about it.