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SDS boiled samples


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#1 DeeDee

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Posted 22 July 2010 - 03:23 AM

Hi,

Can someone tell me if proteins from a complex mixture (like a lysate) can undergo degradation over a period of time after being boiled in laemmli buffer and stored at room temperature?Quite a number of proteases are known to be active in the presence of SDS,is there any chance that proteases can actually refold in the above mentioned condition and cause degradation? Maybe a basic question but haven't been able to get a clear answer until now :P

Thanks,
DeeDee.

#2 Benita

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Posted 27 July 2010 - 05:24 AM

I would say there is no proteolytic activity left after BOILING your sample.




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