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will high-speed centrifuge rupture protein G?

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#1 Curtis


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Posted 21 July 2010 - 11:35 PM

last time I centrifuged my protein G at 2400rpm. but Sigma protocol for my new anti-flag slurry gel suggests over 9000rpm. isn't this too high to break the beads and release bound antibodies?

#2 mdfenko


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Posted 22 July 2010 - 12:19 PM

it depends upon which material the beads are made. but if sigma tells you to spin at 9000 rpm(? not g?) then use what they say.
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