hi,
last time I centrifuged my protein G at 2400rpm. but Sigma protocol for my new anti-flag slurry gel suggests over 9000rpm. isn't this too high to break the beads and release bound antibodies?
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#1
Curtis
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Metaller Scientist
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Excellent
Excellent
Posted 21 July 2010 - 11:35 PM
#2
mdfenko
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an elder
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Excellent
Excellent
Posted 22 July 2010 - 12:19 PM
it depends upon which material the beads are made. but if sigma tells you to spin at 9000 rpm(? not g?) then use what they say.
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genius does what it must
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genius does what it must
i do what i get paid to do
