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will high-speed centrifuge rupture protein G?


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#1 Curtis

Curtis

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Excellent

Posted 21 July 2010 - 11:35 PM

hi,
last time I centrifuged my protein G at 2400rpm. but Sigma protocol for my new anti-flag slurry gel suggests over 9000rpm. isn't this too high to break the beads and release bound antibodies?

#2 mdfenko

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Excellent

Posted 22 July 2010 - 12:19 PM

it depends upon which material the beads are made. but if sigma tells you to spin at 9000 rpm(? not g?) then use what they say.
talent does what it can
genius does what it must
i do what i get paid to do




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