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suitable cell lysis buffer


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#1 sara.r

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Posted 21 July 2010 - 02:01 AM

Hi
I need to do some elisa on my CHO cell line, is there any suitable cell lysis buffer that I can prepare in lab?
regards

#2 bob1

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Posted 21 July 2010 - 01:40 PM

I would imagine that any lysis buffer should work, so long as it solubilises the protein of interest and components don't interfere with the detection system. It would pay to know if the antibody on the ELISA detects native or denatured protein.

Edited by bob1, 21 July 2010 - 01:41 PM.





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