I had a few questions regarding this.
for a positive control would H202 work before fixing, or should I use H202 + Fe (2+)?
Also for anyone that might have experience with this, were the fluorescence increases easily distinguishable?
I have been having a problem with this and can't tell if the fluorescence is just low or if I need to tweak my protocol
fix - methanol 50% or methanol/acetone
DNPH in HCl
block, primary, secondary.
Thanks in advance
Edited by Triwinters, 21 July 2010 - 08:23 AM.