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Recommended poly-HRP for IHC?


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#1 cchase

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Posted 20 July 2010 - 12:03 PM

My lab has been using "ready-to-use" IHC kits for long time (mostly of the Zymo Histo-max variety). These kits include working concentrations of poly-hrp and aec. We are trying to start making our own solutions for IHC now. We have an AEC recipe that seems to work, but we can't find a good poly-HRP antibody that works. So far, we've tried an HRP conjugated secondary that has a single HRP on it as well as a polymer HRP antibody. We can't get any signal (sometimes after 30min incubations with AEC we get difuse background in some tissues) with either of these systems.

Can anyone here recommend a secondary HRP-conjugated antibody that works on thin section IHC of mouse tissues... that you've test and know works?

Thanks.

#2 victorius

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Posted 20 July 2010 - 01:36 PM

I tried the Vector MP-7401 immPress (anti rabbit peroxidase) with Vector AEC immpact substrate. Works nicely for me.

V.

#3 cchase

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Posted 21 July 2010 - 07:10 AM

I tried the Vector MP-7401 immPress (anti rabbit peroxidase) with Vector AEC immpact substrate. Works nicely for me.

V.



We'll look into it. Thanks. It looks like a pretty good deal too compared to other pre-diluted secondaries I've seen.




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