I am making NZY+ broth for a transformation protocol using XL10-gold ultracompetent cells.
My question is: in the recipe, is casamino acids a suitable substitute for NZ amine (casein hydrolysate)? I believe they are considered the same thing, but wanted to get a confirmation.
Thank you.
Bacteria Media (NZY+ broth)
Started by kiikyo, Jul 20 2010 11:44 AM
1 reply to this topic
#1
Posted 20 July 2010 - 11:44 AM
#2
Posted 20 July 2010 - 02:02 PM
casein hydrolysate is nothing more then the milk protein casein that is hydrolysed... so yeah Casamino acids , wich are nothing more then the acids obtained from hydrlosysis of casien is ok. (do keep in mind it does not have tryptophan..)
Edited by pito, 20 July 2010 - 02:03 PM.
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