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Bacteria Media (NZY+ broth)

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#1 kiikyo



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Posted 20 July 2010 - 11:44 AM

I am making NZY+ broth for a transformation protocol using XL10-gold ultracompetent cells.

My question is: in the recipe, is casamino acids a suitable substitute for NZ amine (casein hydrolysate)? I believe they are considered the same thing, but wanted to get a confirmation.

Thank you.

#2 pito



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Posted 20 July 2010 - 02:02 PM

casein hydrolysate is nothing more then the milk protein casein that is hydrolysed... so yeah Casamino acids , wich are nothing more then the acids obtained from hydrlosysis of casien is ok. (do keep in mind it does not have tryptophan..)

Edited by pito, 20 July 2010 - 02:03 PM.

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