This is a silly question, but I wanted to get an honest answer. Are stir bars autoclavable? Or does it depend on the brand (because of this I am not certain). I made media, pH'ed it, and then autoclaved it without removing the stir bar (accident). Do you think it is fine to use as is? Or is it possible that the plastic surrounding the stir bar may "give off" particulates that might be toxic for cells?
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hobglobin
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Posted 20 July 2010 - 07:57 AM
kiikyo, on Jul 20 2010, 05:53 PM, said:
This is a silly question, but I wanted to get an honest answer. Are stir bars autoclavable? Or does it depend on the brand (because of this I am not certain). I made media, pH'ed it, and then autoclaved it without removing the stir bar (accident). Do you think it is fine to use as is? Or is it possible that the plastic surrounding the stir bar may "give off" particulates that might be toxic for cells?
Many (or all?) stir bars are teflon-coated and should be autoclavable, because a teflon-coated frying pan is exposed to much higher temperatures...
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kiikyo
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Posted 20 July 2010 - 08:00 AM
Thank you. I just wanted to make sure. 
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Posted 20 July 2010 - 09:30 AM
I used to do that all the time and never had a problem.
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microgirl
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Posted 20 July 2010 - 11:50 AM
I always autoclave the stir bar in my media so that I can slowly stir it while it cools.
