problems when use qPCR to quantify bisulfite modified DNA
Posted 19 July 2010 - 01:11 PM
I am wondering whether anybody else has seen the same problem. Or what else I can try to fix it. I need to know the accurate DNA concentration!!
Thanks a lot!
Posted 20 July 2010 - 04:50 AM
I hope it can help you a little
Posted 20 July 2010 - 06:47 AM
I am not sure how accurate the nanaodrop can measure the concentration.
Posted 22 July 2010 - 06:30 AM
Posted 22 July 2010 - 09:48 AM
Maybe the values of your standard curve are wrong and thatīs why you get those strange results. From your description I conclude that you perform an absolute quantification running a standrad curve of known concentrations in parallel with your samples. I just wonder how you prepared the DNA for your standard curve and determined the concentrations. Because as far as I know itīs not that easy to measure the concentration of converted DNA because itīs partially single-stranded. So spectrophotometry will not work.