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topoxl cloning

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#1 annc



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Posted 18 July 2010 - 11:29 PM

hi.. all,
can anyone help me....
i have a doubt that.. is it okey doing cloning with topo xl for the genomic DNA which has restricted with RSa1 or Alu1 . The smear concentrating near 1kb. and also what is the faint bands that is there in the smear. whether it will create any problem in further ligation and cloning?

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