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Left polymerase out overnight


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#1 DrNoob

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Posted 18 July 2010 - 01:20 PM

Hello all,
I'm doing one-step RT-PCR and left my polymerase out overnight. It's about 20 degrees celcius in the lab, will my reverse transciptase and taq polymerase still be usable? Thanks.

#2 perneseblue

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Posted 18 July 2010 - 02:57 PM

The taq polymerase is still be usable but I don't know anything about reverse transciptase.
May your PCR products be long, your protocols short and your boss on holiday




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