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Why I am getting this?


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#1 Fhannan

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Posted 18 July 2010 - 03:37 AM

Hello, i hope any one could advise, i have been repeating som many trails all with same results.. why i am getting this sort of migration where the sample migrate obliquely? pleas ei would appreciate any help...

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#2 HomeBrew

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Posted 18 July 2010 - 03:45 AM

The sample is being pulled in the direction of the electric field. If this happens regardless of what the sample is, my guess would be your SDS-PAGE unit is broken. Did you look it over from broken or displaced wires or cracks? Did you try separation of your sample in another unit?




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