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BN-PAGE possible with Tris-glycine


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#1 pizzapop

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Posted 16 July 2010 - 12:37 PM

Hi all, just wondering if anybody knows a protocol for BN-PAGE using Tris-glycine system? All the protocols and papers i read on BN-Page uses Bis-Tris tricine system with pH ~7. If I use a tris-glycine system, should i adjust my pH to 7 as well? Thanks

#2 mdfenko

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Posted 19 July 2010 - 09:39 AM

use the buffer system called for by the protocol.

unless you want to develop your own.
talent does what it can
genius does what it must
i do what i get paid to do




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