I am currently working with a gene expression pattern under two different conditions.
I do get a Ct for my gene around 24, but I can not get a good efficiency on my stantard curve because I only get reading till a 1:50 dilution.
I use cDNA for my standard curve. Can I use genomic DNA for the standard curve? or there is something else I should do?
I have tried very low dilutions (1:5 1:10 1:15 1:20) but as expected the efficiency is not even close to 80%.
Thank you so much!
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very low expression of a sample vs standard curve efficiency
1 reply to this topic
Posted 18 July 2010 - 11:11 PM
if your primers are detecting gDNA (no intron spanning primers) than you can use gDNA for the STD curve, but I would consider to design new primers to improve the efficiency.
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