Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

very simple question about hemocytometer cell counting


  • Please log in to reply
1 reply to this topic

#1 sara.r

sara.r

    sara.r

  • Active Members
  • PipPipPipPipPip
  • 71 posts
0
Neutral

Posted 16 July 2010 - 12:27 AM

hi
I am new in cell culture and I am confused about correct protocol for cell counting using a hemocytometer, actually in our lab some people use the central square of hemocytometer for counting and others count four side squares and divide it to 4. which one is more correct?
best

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,236 posts
336
Excellent

Posted 16 July 2010 - 04:46 PM

The more squares you count, the more accurate your counts will be as counting a single square could result in you counting a square with fewer (or more) cells than average in the solution.

Ideally you should be counting 200-300 cells per side of the haemocytometer, and counting both sides to ensure that your counts are within 15% of each-other (for precision).




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.