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very simple question about hemocytometer cell counting

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#1 sara.r



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Posted 16 July 2010 - 12:27 AM

I am new in cell culture and I am confused about correct protocol for cell counting using a hemocytometer, actually in our lab some people use the central square of hemocytometer for counting and others count four side squares and divide it to 4. which one is more correct?

#2 bob1


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Posted 16 July 2010 - 04:46 PM

The more squares you count, the more accurate your counts will be as counting a single square could result in you counting a square with fewer (or more) cells than average in the solution.

Ideally you should be counting 200-300 cells per side of the haemocytometer, and counting both sides to ensure that your counts are within 15% of each-other (for precision).

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