TaqMan Gene Expression Assays
Posted 16 July 2010 - 12:26 AM
I am working with TaqMan Gene Expression assays. Could someone please tell me what is the max Ct values which are accepted in QPCR? I am working with low cell numbers (50-100) and one of the genes I am looking should be expressed at very low levels. Would you consider Ct values above 35 normal when low gene expression is expected?
ps. I am using rotor gene 6000
Any information would be much appreciated!!
Thank you very much!!
Posted 06 August 2010 - 07:27 AM
or it could be simply considered noise -nucleic acids research doi:10.1093/nar/gkn725
i hope this helps
Posted 08 August 2010 - 11:38 PM