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SDS-PAGE loading dye


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#1 Ardelus

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Posted 15 July 2010 - 11:04 PM

Right, just a quick question here,

I'm running a 15% acrylamide gel and expecting a protein with ~7.5 kDa size. Since I don't want my protein to be running out of the gel I wonder now, will loaded proteins run ahead of the blue loading dye? I'm using 2x loading dye

#2 mole

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Posted 16 July 2010 - 01:25 AM

Hi,

You can reduce the amount of bromophenol blue concentration in Loading buffer that will help you in a better way, As its the concentration of glycerol which affects retention of protein in the well and dye just serve as tracking marker . Iin 15% SDS PAGE dye runs app at 5KDa size.

With Regards,
Mole .




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