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Primary antibody species conversion for IHC/IF?

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#1 victorius



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Posted 15 July 2010 - 08:04 AM

Hello peoples,

I just found they sell online dirt cheap goat anti-rabbit IgG antibodies (unconjugated) and I wonder whether they can be used against primary Abs made in rabbit, so then another Ab (such as Anti-goat FITC) can later be applied to label it. This way I could use -sequentially- 2 primary rabbit Abs after converting the first into a goat one.
I guess an anti-rabbit TRITC may still bind to exposed sites the goat-covered one, but does anybody has any advice?



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