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Mounting Medium for DIG in situ

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#1 cathywill



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Posted 15 July 2010 - 06:14 AM

I am wondering if anyone can recommend a mounting medium for DIG-labelled in situ.

Since the DIG will give a blue signal, I think the DAPI mounting medium usually used in my lab might not be that good. I am thinking of using a HE stain to distinguish the cells. Anyone has good suggestions?

Thank you!

#2 bob1


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Posted 15 July 2010 - 06:50 PM

The DIG colour is blue to the eye, as fas as I know it is not fluorescent, so mounting in DAPI wouldn't alter DAPI fluorescence. You could mount them in standard DPX or some other colour neutral mounting medium.

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