direct lysis of cell pellet
Posted 15 July 2010 - 01:22 AM
I tried to directly lyse my cell pellets in a buffer containing 8M urea by incubating at RT for 1h. But when I tried to load my samples on SDS-PAGE, the solution was far too sticky. In my opinion this is due to DNA.
I have already tried centrifugation to pellet the DNA and passing the solution through a needle and both did not work. I do not want to heat my samples because of the urea. Any additional ideas how to get rid of the DNA?
Thanks in advance
Posted 18 July 2010 - 05:50 PM
have u tried using QIAgen shredder? It will help to get rid of the DNA but its kinda time-consuming though..
Posted 19 July 2010 - 09:40 AM
urea will decompose and carbamylate the protein.
y cant u heat becos of the urea?
genius does what it must
i do what i get paid to do