All protocols I have found for hybidoma production start with female Balb/c mice. The Balb/cI understand is required to prevent rejection if an ascites step is used, but why female mice?We are planning some experiments with knockout mice and it would be great to know if thereare hazards in trying to also use male mice. Everyone uses female mice only but no one Ihave asked knows why.
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anonymous
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Posted 24 October 2000 - 09:00 PM
The answer has simple: male BALB/c fight and kill each other. Females co-habitate well and for long-term immunization studies, you simple cannot use male mice.
