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Positive control for Northern Blot


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#1 Xavier

Xavier

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Posted 14 July 2010 - 10:39 AM

Hi everyone,

my boss ask me to have a good positive control for my northern blot.
I did a lot of lecture but I just saw negatif control.
So do you have a idea of what's a good controlo for a northern?

thanks

#2 bob1

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Posted 15 July 2010 - 06:54 PM

Good positive control is something that you know contains the RNA sequence that you are looking for in your northerns. This could be from a cell line transfected with a plasmid.




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