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C2C12 differentiation


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#1 laurequillo

laurequillo

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Posted 13 July 2010 - 05:49 AM

Ey guys, a general question:

I want to differentiate my C2C12 cells to muscle cells. Normally I just put them in medium with 2% horse serum. The problem is that normally I can not go more than 5-6 days in the differentiation proccess because they start to deattach, and to die. Do you have the same problem? any suggestion?
Thanks
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