I wish to do the proteasome assay using the flouregenic substrates Suc-LLVY-AMC, Z-LLE-AMC, Boc-Leu-Arg-Arg-AMC. All the protocols i have read are using well plates. But my spectroflourometer does not have a plate reader attachment. i want to upscale the assay to the cuvette. will diluting the same amounts as required in wel plates with the assay buffer give me the reading??? also what are the other precautions i must keep in mind while working up.. If anybody can provide me with a detailed protocol it would be really really helpful
Edited by poonam thakur, 13 July 2010 - 05:40 AM.