Hi! I'm having some problems with PMA-differentiated U937 cells. Although I can induce IL-10 production with my stimulus, spontaneous secretion also raises in a time-dependent fashion... This doesn't happen with TNF-alpha.
I suspect of FCS in the complete medium (+RPMI+PenG)...
Does anyone has a similar experience?
Thanks in advance.
Spontaneous Il-10 with U937 cell line
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