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Spontaneous Il-10 with U937 cell line


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#1 K-Ag

K-Ag

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Posted 13 July 2010 - 02:57 AM

Hi! I'm having some problems with PMA-differentiated U937 cells. Although I can induce IL-10 production with my stimulus, spontaneous secretion also raises in a time-dependent fashion... This doesn't happen with TNF-alpha.

I suspect of FCS in the complete medium (+RPMI+PenG)...

Does anyone has a similar experience?

Thanks in advance.




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