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protein extraction


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#1 SF_HK

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Posted 13 July 2010 - 12:20 AM

HI,

I'm new to protein extraction. I had extracted proetin from cell lines using ripa buffer. Now I need to extract proetins from tumor tissues and soem protocols recommend Urea buffer. What is the difference and is it alright to extract protein from tumor tissues using ripa?

#2 jasmina

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Posted 15 July 2010 - 06:03 AM

i think the difference is the solubilization of your proteins!
I used ripa buffer to solubilize one membrane protein! when i used buffer with urea, i couldnt solubilize better and see by western blot.
also, im not sure, but it may depends on what you want to do as experiment after.




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