question on buffering a solution
Posted 12 July 2010 - 08:24 AM
Say I am bringing up a solution (ex: Tris) to a certain pH, say 7.5 with HCl, and accidentally the pH overshoots to 7.0, or even 6.5. Would it OK if I add NaOH to bring the pH up to 7.5? I've read somewhere that whenever the pH overshoots, throw away the buffer and re-make it fresh. What are your opinions? People said that it totally depends upon the buffer. What is the conceptual difference?
Posted 12 July 2010 - 08:38 AM
Normally it shouldnt matter because you neutralise one with the other one.
Posted 12 July 2010 - 10:56 AM
if you can't tolerate salt and you overshoot then you can add more tris base, titrate then adjust to the new final volume.
you can also just use equimolar tris base and tris-hcl to prepare the buffer (final volume doesn't matter).
genius does what it must
i do what i get paid to do
Posted 12 July 2010 - 11:00 AM
oh mdfenko already answered it perfectly...anyway it's a matter of empiricism, i.e. try it out...
Edited by hobglobin, 12 July 2010 - 11:06 AM.
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 12 July 2010 - 06:42 PM