problem about fused protein
Posted 11 July 2010 - 10:42 PM
I am constructing a vector---Promoter::GFP-protein
The protein will be fuctioned as a marker gene together with GFP.
My question is whether it is necessary to add the ATG after the GFP coding sequence and 3'UTR of the protein to the end of the protein coning sequence.
Thanks very much!
Posted 13 July 2010 - 06:06 PM
You don't need the UTR as far as I know.
Posted 14 July 2010 - 12:42 AM
Well, it depends if you want to see degradation of your mRNA, or translational inhibition... but I guess you don't.