horrific western blots , please help
Posted 11 July 2010 - 03:14 PM
i'm at my wits end with these. any advice will be greatly appreciated
Posted 11 July 2010 - 03:26 PM
Posted 11 July 2010 - 03:37 PM
It looks like there is too much salt in the loaded sample, which can result in the bands getting distorted like that, though this doesn't account for your good result with the same samples, unless you used a different batch of loading buffer or something. It could also be that the pre-cast gels are going off or the the buffer composition not being quite right (especially if you re-use buffer).
hi thank you for your prompt reply. unfortunately, i used loading buffer from the same batch. gels dont expire until september and I ran the samples that worked well a day before these, hence buffer composition change is an unlikely suspect..