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Primer design for qPCR

Started by borriello.87, Jul 09 2010 04:52 AM

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2 replies to this topic

#1 borriello.87

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Posted 09 July 2010 - 04:52 AM

Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics... <_<

Thanks!

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#2 PhilipV

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Posted 13 July 2010 - 03:08 AM

Hello,

I did a lot of qPCR work lately and I had very good results with the rtprimerdb (http://medgen.ugent.be/rtprimerdb/) and primerquest (http://eu.idtdna.com...st/Default.aspx) tools.

With rtprimerdb, you can, however, design only 5 primer pairs per 24 hours.

If you have any other problems, feel free to let me know!

Regards,
Philip

borriello.87, on Jul 9 2010, 02:52 PM, said:

Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics...

Thanks!

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#3 calou

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Posted 07 March 2012 - 12:14 AM

I can suggest you this one "getprime": http://updepla1srv1.epfl.ch/getprime/ It combines all features you need to have good primer quality and specificity (do not need to blast!). I get very good primers with this design.

Good luck!