Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primer design for qPCR


  • Please log in to reply
2 replies to this topic

#1 borriello.87

borriello.87

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 37 posts
0
Neutral

Posted 09 July 2010 - 04:52 AM

Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics... <_<
Thanks!

#2 PhilipV

PhilipV

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 13 July 2010 - 03:08 AM

Hello,

I did a lot of qPCR work lately and I had very good results with the rtprimerdb (http://medgen.ugent.be/rtprimerdb/) and primerquest (http://eu.idtdna.com...st/Default.aspx) tools.

With rtprimerdb, you can, however, design only 5 primer pairs per 24 hours.

If you have any other problems, feel free to let me know!

Regards,
Philip

Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics... :o
Thanks!



#3 calou

calou

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 07 March 2012 - 12:14 AM

I can suggest you this one "getprime": http://updepla1srv1.epfl.ch/getprime/ It combines all features you need to have good primer quality and specificity (do not need to blast!). I get very good primers with this design.

Good luck!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.