could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics...
Thanks!
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2 replies to this topic
#1
borriello.87
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Posted 09 July 2010 - 04:52 AM
Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics...
Thanks!
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics...
Thanks!
#2
PhilipV
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Posted 13 July 2010 - 03:08 AM
Hello,
I did a lot of qPCR work lately and I had very good results with the rtprimerdb (http://medgen.ugent.be/rtprimerdb/) and primerquest (http://eu.idtdna.com...st/Default.aspx) tools.
With rtprimerdb, you can, however, design only 5 primer pairs per 24 hours.
If you have any other problems, feel free to let me know!
Regards,
Philip
I did a lot of qPCR work lately and I had very good results with the rtprimerdb (http://medgen.ugent.be/rtprimerdb/) and primerquest (http://eu.idtdna.com...st/Default.aspx) tools.
With rtprimerdb, you can, however, design only 5 primer pairs per 24 hours.
If you have any other problems, feel free to let me know!
Regards,
Philip
Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics...
Thanks!
#3
calou
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Posted 07 March 2012 - 12:14 AM
I can suggest you this one "getprime": http://updepla1srv1.epfl.ch/getprime/ It combines all features you need to have good primer quality and specificity (do not need to blast!). I get very good primers with this design.
Good luck!
Good luck!
