Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

the best loading control throughout cell cycle


  • Please log in to reply
No replies to this topic

#1 iamdhan

iamdhan

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 09 July 2010 - 01:52 AM

Hi,

Which protein could be a reliable loading control when your topic protein's expression level might be changeable?

Tubulin was what I used, but I've read from some paper that it is not stably synthesized during the cell cycle, so that's out of the option Iguess...

I specifically want to check if my protein plays a role in S or G2 phase.

As of now, Actin is my new loading control for sample from cytosol and Lammin for nuclear extracts.

Am I doing it right? Would I have some better choice?


I haven't had much experience in this feild, so my question could sound so stupid to you.

Any suggestion will help!

Thx.

Edited by iamdhan, 09 July 2010 - 02:55 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.