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the best loading control throughout cell cycle

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#1 iamdhan



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Posted 09 July 2010 - 01:52 AM


Which protein could be a reliable loading control when your topic protein's expression level might be changeable?

Tubulin was what I used, but I've read from some paper that it is not stably synthesized during the cell cycle, so that's out of the option Iguess...

I specifically want to check if my protein plays a role in S or G2 phase.

As of now, Actin is my new loading control for sample from cytosol and Lammin for nuclear extracts.

Am I doing it right? Would I have some better choice?

I haven't had much experience in this feild, so my question could sound so stupid to you.

Any suggestion will help!


Edited by iamdhan, 09 July 2010 - 02:55 AM.

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