Which protein could be a reliable loading control when your topic protein's expression level might be changeable?
Tubulin was what I used, but I've read from some paper that it is not stably synthesized during the cell cycle, so that's out of the option Iguess...
I specifically want to check if my protein plays a role in S or G2 phase.
As of now, Actin is my new loading control for sample from cytosol and Lammin for nuclear extracts.
Am I doing it right? Would I have some better choice?
I haven't had much experience in this feild, so my question could sound so stupid to you.
Any suggestion will help!
Edited by iamdhan, 09 July 2010 - 02:55 AM.