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Primer design for qPCR


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#1 borriello.87

borriello.87

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Posted 09 July 2010 - 01:08 AM

Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics... B)
Thanks!

#2 ivanbio

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Posted 09 July 2010 - 07:27 AM

Designing qPCR primers should be as easy as using the software that comes with your qPCR instrument. If you do not have access to such software, you can use software available online, like IDT's:

http://www.idtdna.co...ns/RealTimePCR/

There are also online tutorials on what you need to consider, like this one from Premier Biosoft:

http://www.premierbi...mer_Design.html

Good luck

Ivan
Carlsbad, CA




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