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How to grow J774 in suspension


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#1 Pheobe

Pheobe

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Posted 08 July 2010 - 11:55 PM

Hi everybody.

This might be a dumb question, but can anybody give me a protocol how to grow and subculture J774 in suspension. I'm only used to aderent cultures, but as many of you have stated the macrophages stick to the plastic like superglue. Now I want to try the stirrer bottles but have never done that before. So can anyone help me?

Thank You B)
Pheobe

#2 rhombus

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Posted 09 July 2010 - 01:34 AM

Dear Pheobe,

I have posted many times on J774. If you use the search option and insert J774...many of my posts will come up. For example see below:-



http://www.protocol-...e...084&hl=J774




If you need any further information please send me a PM.


Kindest regards


Uncle Rhombus




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