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Growth conditions for T cellline


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#1 anonymous

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Posted 28 November 2001 - 10:00 PM

Hi, could anybody direct me to reference or web site that describes the proper growth conditions for T cell line (stimulation, rest and media supplements)Thanks.

#2 Marina

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Posted 09 February 2002 - 01:51 PM

Why won't you try going to the website of the supplier?  They usually provide you with such info.

#3 boris pufong

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Posted 12 February 2002 - 03:48 AM

growing Thp1 cells, i use RPMI 1640 medium (425ml)supplimented with 10% FCS(10ml), 1%pen/strip optional(5ml,50Unit/ml), 15ml Hepes (30mM) and 5ml L-glutamine




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