I'm trying to design primers using NCBI Primer-BLAST. I'm amplyfing from mouse genomic DNA and want to check if my primers are specific, so under "Primer Pair Specificity Checking Parameters" I selected mus musculus as the organism and "Genome (reference assembly form selected organisms)" as the database to check against. Now, the website says that mouse is definitely one of the "selected organisms" included in that database. Nevertheless, I always get the following error:
Has anybody experienced this? Any tips for a fix/workaround? It works if I choose to check specificity on the genomes of all organisms, but the search takes much longer then.