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What means BamHI-linkered

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#1 lisboa



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Posted 08 July 2010 - 08:47 AM

Hi everyone,

I need to do a remove an insert for a plasmid. I only have the description of the restriction sites from a paper, no complete sequence. And on the paper, it is written "BamI-likered" about a site. Could you help me , because I don't know what this means...:(

#2 bob1


    Thelymitra pulchella

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Posted 08 July 2010 - 05:34 PM

I would guess that they used a BamH1 link to create a compatible restriction site for cloning the target sequence. This will mean that there was a BamH1 site followed by a short (up to 20 bp) sequence of bases ligated to the target sequence.

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