Whole mouse brain storage
Posted 08 July 2010 - 04:55 AM
I will be removing whole mouse brains to be later used for western blotting. I was planning on removing the brain, immediately flash freezing it in isopentate, then storing the frozen brain at -80 C. When I need the brain for western blotting, I plan on thawing the brain on ice or ice cold PBS, then dissect out the hippocampus. Has anyone done something similar to this before? If so, what did you do? Also, does anyone see anything wrong with my plan? Thanks for the help!
Posted 08 July 2010 - 05:38 AM
If its just the hippocampus you need why don't you just dissect out that region when you remove the brain and then freeze the required region in LN2 for -80 storage. As long as you can remove the brain and do your dissections on ice (I usually dissect the brain on the surface of a glass petri dish filled with ice to maintain the cold temp for longer) within 5-7 min, the tissue will be fine.
Posted 08 July 2010 - 06:43 AM
Posted 10 August 2010 - 02:48 PM