Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Pipettors for Real Time PCR (urgent)


  • Please log in to reply
5 replies to this topic

#1 Adrian K

Adrian K

    Legendary Graduate Beggar

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 708 posts
28
Excellent

Posted 07 July 2010 - 08:09 PM

Dear All,
I plan to do real-time PCR work and I do need an accurate pipettor for me to do my work and consider to get one complete set for myself.

Do you have any good and recommended brands?

I am considering getting HTL DISCOVERY Comfort, Thermofisher or Eppendorf . Any comments?
Thanks.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,805 posts
133
Excellent

Posted 08 July 2010 - 06:47 AM

pipetman and eppendorf are probably the best out there.

i use eppendorf "reference" pipets. i prefer the way they feel in my hand over those that use a pipetman form factor. they cost a little more but the comfort is worth it (to me).
talent does what it can
genius does what it must
i do what i get paid to do

#3 Adrian K

Adrian K

    Legendary Graduate Beggar

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 708 posts
28
Excellent

Posted 11 July 2010 - 07:32 PM

Thanks mdfenko. You are always here to reply me. :P I do agree with you. Now lets see how is the price I can get from my supplier.

Anyone else got any other comments?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#4 than4

than4

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 36 posts
3
Neutral

Posted 13 July 2010 - 10:31 PM

When we bought pipettes earlier this year we were able to get some on trial for a week or so. If you could do this to see how you like working with them, I would. Failing that perhaps you could have a look around other labs in your department or institution and borrow or play with someone else's pipettes to see if you like them. Comfort and accuracy are most important and seem to be quite a personal thing.

I don't do RT-PCR but the others in my lab like the Eppendorf or the Finn pipettes. The only hassle with the Eppendorf ones is that they will only take Eppendorf tips, which becomes quite expensive.

I also have an anti-recomendation against the CAPP pipettes, a former post-doc got them very cheap, but they are heavy, hard to use and inaccurate.

There was another general pipette thread here recently, perhaps read that too.

#5 nightingale

nightingale

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 196 posts
7
Neutral

Posted 14 July 2010 - 02:00 AM

i agree with mdfenko, concerning the Eppendorf pipette as a good choice
and how it feels also :)
" The more you learn, the more you realize how little you know ... "

#6 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,805 posts
133
Excellent

Posted 14 July 2010 - 06:14 AM

The only hassle with the Eppendorf ones is that they will only take Eppendorf tips, which becomes quite expensive.

we use tips from usa scientific for our eppendorfs. we can also use tips from other vendors (eg denville). we use the best quality tips for which we get a good price.

the only eppendorf brand tips that we use are the ones that came with the pipettes.
talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.