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Internal control


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#1 saraarasus

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Posted 07 July 2010 - 04:04 PM

Hi,
I did western for an antigen secreted in the media. I collected the supernatan, loaded the same amount of protein in each well and did the western and ELIZA.
The reviewer is asking me for internal control.
Whar internal control you think I can use?

Thanks,
Sara

#2 Inmost sun

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Posted 13 July 2010 - 01:26 AM

Hi,
I did western for an antigen secreted in the media. I collected the supernatan, loaded the same amount of protein in each well and did the western and ELIZA.
The reviewer is asking me for internal control.
Whar internal control you think I can use?

Thanks,
Sara


the protein of interest in purified or at lest enriched amounts loaded on the same gel or ELISA plate




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