Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Adding stabilizer to antigen increases signal


  • Please log in to reply
1 reply to this topic

#1 seriema

seriema

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 07 April 2002 - 12:38 PM

We carry out sandwich-ELISA with different antigens (bacteria, viruses, toxins).
To stabilise bacterial antigen, we add BSA to it. The solution is stable for the whole day and the signal of this solution is even higher than before.
But this works also with other antigens, which actually do not need stabilisation, like toxins.
Could it be, that BSA has a stabilising effect on the detection antibody?
Thatís how we carry the ELISA out:
chemical Immobilisation of the capture antibody
Blocking with BSA
Antigen
Detection-antibody, (with BSA and detergent)
For the adsorptive immobilisation it is recommended in some protocols to add BSA to the capture-antibody solution.
Is this the same effect? Is there a reason why we shouldnít add BSA to any kind of antigen?

#2 leahfuller

leahfuller

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 26 April 2002 - 11:52 AM

i thought that BSA blocked non-specific binding, therefore the specific signal would appear better...




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.