2 replies to this topic

[sara.r]

Hi
I am new in elisa and I get confused about different types of 96 well plates, I know flat shaped wells are more suitable but I am not sure what brand is better, I read something that protein binding is depend on the type of material that is used in plates, so please help me to choose a suitable kind of plate, by the way I am going to set up elisa on crude cell mammalian lysate.

regards

[zodiac1505]

sara.r, on Jul 7 2010, 06:55 AM, said:

Hi
I am new in elisa and I get confused about different types of 96 well plates, I know flat shaped wells are more suitable but I am not sure what brand is better, I read something that protein binding is depend on the type of material that is used in plates, so please help me to choose a suitable kind of plate, by the way I am going to set up elisa on crude cell mammalian lysate.

regards

I have been doing ELISA for a while though I look at cell culture supernatant. We use flat bottom 96 well plates from nunc. Maybe you can try those. I have never tried any other brands but this seems to work very well for me.

[sgt4boston]

In a 'side by side' experiment years ago, I examined nunc and others...for 'sandwhich type' immunoassays nunc appear to have the best plates...followed by corning and others. If you can find one of the nunc's power point presentations on-line it would be very helpful to you.

there are also these new plates: http://www.anptinc.com/elisa.html you can try.

Most companies will provide free samples. Use the plates with individual strips to save $