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Antigenicity of GST-tag.


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#1 Jack Dirri

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Posted 05 June 2002 - 08:49 AM

Hi,
I produced polyclonal antibody against GST fusion protein. The polyclonal antibody that I got has a good specificity to my protein, but react poorly with GST protein. Does anybody know why GST is not a good antigen or have the same experience? Thanks in advance.

#2 dpiccini

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Posted 28 May 2003 - 11:26 AM

Hi
I usually produce monoclonal antibodies using GST fusion protein; in my experience, GST is not so immunogenic, even after multiple injections of the recombinant protein; the GST we have in the fusion proteins is from Schistosoma j.




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