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MTT Assay Sensitivity


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#1 cells

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Posted 06 July 2010 - 12:08 PM

Hi,

I just have a quick question; I've recently started doing MTT assay and I'm currently dissolving the MTT crystals in DMSO and leaving it on a shaker at 37oC for ~15 minutes. I'm just wondering if there would be any drastic changes to sensitivity of the assay between different samples if it was left overnight or for a longer period of time.

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#2 Curtis

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Posted 07 July 2010 - 02:38 AM

yes it would be, because we are talking about cells. do you plate your cells in 96-well plate?

#3 cells

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Posted 07 July 2010 - 06:56 AM

No, they're done in a 24 well plate at the moment.




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