Hi,
I just have a quick question; I've recently started doing MTT assay and I'm currently dissolving the MTT crystals in DMSO and leaving it on a shaker at 37oC for ~15 minutes. I'm just wondering if there would be any drastic changes to sensitivity of the assay between different samples if it was left overnight or for a longer period of time.
Thanks
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MTT Assay Sensitivity
Started by cells, Jul 06 2010 12:08 PM
2 replies to this topic
#2
Curtis
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Posted 07 July 2010 - 02:38 AM
yes it would be, because we are talking about cells. do you plate your cells in 96-well plate?
#3
cells
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Posted 07 July 2010 - 06:56 AM
No, they're done in a 24 well plate at the moment.
