Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Frozen cells in -80


  • Please log in to reply
1 reply to this topic

#1 Aravin

Aravin

    member

  • Active Members
  • Pip
  • 14 posts
1
Neutral

Posted 06 July 2010 - 05:55 AM

Hello,

I froze some cells in May and I put them in -80 fridge. Usually I transfer them to liquid nitrogen storage the next day but I seem to have completely forgotten about them until now. Can I still transfer them to liquid nitrogen or is it too late? If it's too late, why?

Thanks.

#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,112 posts
58
Excellent

Posted 06 July 2010 - 08:30 AM

I always save my cells at -80C because I can immediately find them in the freezer. Liquid nitrogen tanks are always packed with cryotubes and hard to find what you want.however I don't recommend it to you :D....but anyway, yes, you can use it now or put it into nitrogen. I have used cells that have been in -80 for nearly 2 years. many cells didn't survive but still I could revive them.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.