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Frozen cells in -80

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#1 Aravin



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Posted 06 July 2010 - 05:55 AM


I froze some cells in May and I put them in -80 fridge. Usually I transfer them to liquid nitrogen storage the next day but I seem to have completely forgotten about them until now. Can I still transfer them to liquid nitrogen or is it too late? If it's too late, why?


#2 Curtis


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Posted 06 July 2010 - 08:30 AM

I always save my cells at -80C because I can immediately find them in the freezer. Liquid nitrogen tanks are always packed with cryotubes and hard to find what you want.however I don't recommend it to you :D....but anyway, yes, you can use it now or put it into nitrogen. I have used cells that have been in -80 for nearly 2 years. many cells didn't survive but still I could revive them.

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