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[laurequillo]

Hey guys!

I am having a problem in my cell culture. Everyother time I infect cells with a given shRNA some of the plates I infected get contaminated. They became smelly and with a cloudy/white medium. I would say it is a bacterial contamination (it is too smelly).
The point is that two different people used this plasmid to produce virus (so different hands, different batches of virus), with different cells, in different days but always using the same incubator (S2 incubator). And both of them found that some plates had that kind of contamination. But it doesnt happen everytime, lets say 1 of every 3 times. And normally the "contamination" appears like 2 days after you add the virus. (24h with the virus, then you add Puromycin, and 24 h after the puromycin it appears)
My question is, could it be because the virus?? too much virus maybe? could they produce the lysis of the cells, and the odor/contamination? Or it is maybe the incubator? (it was cleaned already just in case...), or the Puro (not likely because it doesnt happen in all the plates, and not always)

What do you think?

Thanks!