Posted 04 July 2010 - 08:47 AM
Hi Ismail,
I was having same problem like you before. Same like you, the problem doesn't happen to rabbit, horse, sheep and human blood when I was working with them. I haven't solve the problem yet (as I had other project in hand now), but I do think maybe is due to the osmotic pressure in our buffer (not isotonic enough to the cells). I was thinking of increase the salt concentration in the washing buffer, but I haven't try it yet as I had lack of time.
Anybody got similar experiences or advices?
Adrian.
P/S: my washing and suspension buffer is PBS.
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