Restriction Digest Question
Posted 04 July 2010 - 01:21 AM
I have two sequences of about 700bp, and I would like to see if a particular RFLP enzyme can differentiate them from each other on a gel. Is there a programme that I can use to tell me which enzyme I should use? I am currently tackling NEBcutter but not having much luck.
Posted 07 July 2010 - 07:00 AM
I did search for enzyme distinguishing my wild-type and mutant gene like this.
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