Hello everyone.
I would like to know how to calculate the efficiency of the qPCR. The slope value is -3.46. Please help
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tea-test
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Posted 05 July 2010 - 05:24 AM
hi,
the formula of the efficiency (in %) is E(%) = (10-1/slope -1) *100. so for your slope this should be 94.5 % which is pretty good
the formula of the efficiency (in %) is E(%) = (10-1/slope -1) *100. so for your slope this should be 94.5 % which is pretty good
tea-test: The artist formerly known as Ned Land
