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High pI proteins


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4 replies to this topic

#1 proteinz

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Posted 03 July 2010 - 04:47 AM

Hi guys,
I am looking for a bit of help finding proteins which have a pI above 8 (preferably >9) for a experiment. Does anybody know any? Particularly any which could be bought lyophilised from sigma etc. Source (Organism) not NB!

Thanks to all in advance for your help. It's taking me ages to fnd sequences off the top of my head and calculate the theoretical pI!

#2 K.B.

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Posted 03 July 2010 - 11:20 PM

Trypsinogen from bovine pancreas - Isoelectric focusing marker, pI 9.3 - Sigma - T1146

Lectin from Lens culinaris (lentil) - Isoelectric focusing marker, pI (1) 8.2, (2) 8.6, (3) 8.8 - Sigma - L1277

#3 doxorubicin

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Posted 04 July 2010 - 06:33 PM

What about avidin?.....pI is 10.5 and lots of companies sell it.

#4 mdfenko

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Posted 07 July 2010 - 07:56 AM

there are also the histones. they have high pI's.

Edited by mdfenko, 07 July 2010 - 07:57 AM.

talent does what it can
genius does what it must
i do what i get paid to do

#5 proteinz

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Posted 08 July 2010 - 02:02 PM

Thanks guys! V helpful!




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