Sometimes we end up aspirating a large amount when doing tissue-culture. We need a disinfectant to be used in the vacume flask durring the day, but we also want to autoclave the mixture without dammaging the stainless steel. I am pretty sure that chlorox (Sodium Hypochlorite) will tarnish the autoclave very quickly, but I am looking into using Chloramine or Benzalkonium Chloride. Has anyone autoclaved these chemicals at, say .2% Benzalkonium or 5%Chloramine? Maybe someone knows of an effective disinfectant that is stable or minamally corrosive at autoclaving condidtions?
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2 replies to this topic
#2
perneseblue
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Posted 03 July 2010 - 09:08 PM
Chloramine tarnishes steel, do not autoclave.
Looking at its chemical structure, Benzalkonium chloride looks okay in an autoclave. It works by being a surfactant, not an oxidizing agent. Maybe somebody else can comment.
Looking at its chemical structure, Benzalkonium chloride looks okay in an autoclave. It works by being a surfactant, not an oxidizing agent. Maybe somebody else can comment.
May your PCR products be long, your protocols short and your boss on holiday
#3
bob1
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Thelymitra pulchella
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Excellent
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Posted 06 July 2010 - 05:24 PM
Why not just aspirate and then autoclave without disinfectant? Do not use virkon or trigene if you are autoclaving after aspirating.
